For the second chemostat experiment

For the second chemostat experiment

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Typhimurium for four consecutive periods of 10 days (720 generations), alternating between the two hosts.For the second chemostat experiment, the fitness and attachment rates of each viral population were measured using qPCR in liquid culture in order to identify and characterise fitness costs associated with host switching. Deep sequencing of chemostat samples was also carried out to identify allelic changes occurring before and after host switches. Viral samples were chosen to capture substitutions associated with each host across the experiment (which might explain observed changes in fitness) and time series were picked to identify the dynamics of adaptation on a new host.

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